He aha nā paipu hōʻiliʻili koko EDTA a pehea e hoʻohana ʻia ai?

Ma ka hoʻāʻo ʻana i ka lāʻau lapaʻau a me ka maʻi lapaʻau a me ka mālama ʻana,ʻO nā paipu hōʻiliʻili koko EDTA, e like me nā mea hoʻohana kī no ka hōʻiliʻili koko, pāʻani i kahi hana koʻikoʻi i ka hōʻoia ʻana i ka pono o nā laʻana a me ka pololei o ka hoʻāʻo. Ma kēia ʻatikala, e loiloi piha mākou i kēia "mākua ʻike ʻole" i ka lāʻau lapaʻau mai nā ʻano o ka wehewehe ʻana, ka waihoʻoluʻu kala, ka loina anticoagulation, ke kumu hoʻāʻo a me ka hoʻohana maʻamau.

He aha kaEDTA paipu ohi koko?

ʻO ka pahu hōʻiliʻili koko ʻo EDTA kahi ʻano o ka ʻōpala ʻohi koko i loaʻa iā Ethylene Diamine Tetraacetic Acid a i ʻole kona paʻakai, ka mea i hoʻohana nui ʻia no ka hōʻiliʻili ʻana i nā laʻana koko a me ka mālama anticoagulant. Hiki i ka EDTA ke ālai i ka coagulation cascade reaction ma ka chelating i nā ion calcium i loko o ke koko, i mea e mālama ai i ke koko i loko o kahi wai no ka manawa lōʻihi, a hāʻawi i nā laʻana paʻa no nā hoʻokolohua o ke koko maʻamau a me ka biology mole. Hāʻawi ia i nā laʻana paʻa no ke koko maʻamau, molecular biology a me nā hoʻokolohua ʻē aʻe.

Ma keʻano he mea nui onā mea hoʻohana lapaʻau, Pono nā paipu hō'ili'ili koko EDTA e ho'okō i ke kūlana 'āina o "Single-use venous blood sample collection containers" (eg GB/T 19489-2008) e hō'oia i ka hana o sterility, non-pyrogenic a me non-cytotoxicity.

Nā waihoʻoluʻu like ʻole o nā paipu hōʻiliʻili koko EDTA

E like me nā kūlana maʻamau o ka honua (e like me nā alakaʻi CLSI H3-A6), hoʻopaʻa mau ʻia nā paipu hōʻiliʻili koko EDTA i ka poni (EDTA-K2/K3) a i ʻole polū (sodium citrate i hui pū ʻia me EDTA) e hoʻokaʻawale i ka hoʻohana.

'Ōlelo Aʻo: Hiki ke hoʻopaʻa inoa ʻia kekahi mau hōʻailona i nā kala ʻē aʻe, e nānā i nā kuhikuhi ma mua o ka hoʻohana ʻana.

ʻO ka hana anticoagulation o nā paipu hōʻiliʻili koko EDTA

ʻO EDTA ma o kāna pūʻulu carboxyl molecular (-COOH) a me nā ion calcium i loko o ke koko (Ca²⁺) i hui ʻia e hana i kahi chelate paʻa, no laila ke keʻakeʻa nei i ka hoʻāla ʻana o ka plasminogen, ke kāohi nei i ke kaʻina coagulation o fibrinogen i fibrin. Loaʻa kēia anticoagulation i kēia mau ʻano:

1. ka hoʻomaka wikiwiki o ka hana: hiki ke hoʻopau i ka anticoagulation i loko o 1-2 mau minuke ma hope o ka hōʻiliʻili koko;

2. paʻa kiʻekiʻe: hiki ke mālama ʻia nā laʻana no nā hola ʻoi aku ma mua o 48 (hiki ke hoʻonui ʻia i ka refrigerated i nā hola 72);

3. Ka laulā o ka noi: kūpono no ka hapa nui o nā ho'āʻo hematology, akā, ʻaʻole no ka coagulation a i ʻole nā ​​hoʻokolohua hana platelet (pono ʻia nā paipu sodium citrate).

Nā mea hoʻāʻo kumu o ka paipu hōʻiliʻili koko EDTA

1. ka nānā 'ana i ke koko ma'amau: ka helu ke'oke'o ke'oke'o, nā palena koko 'ula'ula, ka no'ono'o hemoglobin, a pēlā aku;

2. ʻike pūʻulu koko a me ka hoʻohālikelike ʻana i ke keʻa: hui koko ABO, ʻike kumu Rh;

3. ʻike molecular: hoʻāʻo genetic, hoʻoholo i ka loaʻa viral (e laʻa me HIV, HBV);

4. hemoglobin glycated (HbA1c): ka nānā ʻana i ka glucose koko lōʻihi no ka maʻi diabetes mellitus;

5. ka nānā 'ana i ka parasite koko: Plasmodium, ka 'ike microfilariae.

Hoʻohana i nā mea maʻamau a me ka mālama ʻana

1. Kaʻina ohi:

Ma hope o ka hoʻomaʻemaʻe ʻana i ka ʻili, e hana e like me ke ʻano o ka hōʻiliʻili koko venous;

Ma hope koke o ka hōʻiliʻili ʻana, e hoʻohuli i ka paipu hōʻiliʻili koko 5-8 mau manawa e hōʻoia i ka hui pū ʻana o ka anticoagulant me ke koko;

Hōʻalo i ka haʻalulu ikaika (e pale i ka hemolysis).

2. Ka waiho ʻana a me ka lawe ʻana:

E kūʻai ma ka lumi wela (15-25 ° C), e pale i ka wela a i ʻole ka hau;

E kau pololei i ka wā o ka lawe ʻana e pale i ka hemo ʻana o ka pāpale paipu.

3. nā hiʻohiʻona contraindication:

Pono nā paipu sodium citrate no ka Coagulation IV (PT, APTT, etc.);

Pono ka ho'āʻo hana platelet i ka paipu sodium citrate.

Pehea e koho ai i ke ʻano kiʻekiʻeEDTA paipu ohi koko?

1. Ka hōʻoia a me ka hōʻoia: koho i nā huahana i hala i ka ISO13485 a me ka hōʻoia CE. 2;

2. Palekana waiwai: pono ke kino o ka paipu me ka ʻole o ke koena plasticizer;

3. ʻO ka hoʻopaʻa ʻana pololei: ʻo ka nui o ka anticoagulant i hoʻohui ʻia e like me ke kūlana aupuni (e laʻa me EDTA-K2 ka nui o 1.8±0.15mg/mL);

4. Kaulana inoa: Hāʻawi ʻia ka mea nui i nā mea kaulana kaulana i ke kahua o nā lāʻau lapaʻau e hōʻoia i ka paʻa ʻana o ka hui.

Ka hopena

Ma keʻano he lālā kī omea lawe koko, ʻO nā paipu hōʻiliʻili koko ʻo EDTA he hopena pololei i ka pololei o nā hopena hoʻāʻo e pili ana i kā lākou waiwai anticoagulant. Ma ka hoʻohālikelike ʻana i ka hoʻohana ʻana i nā paipu hōʻiliʻili koko kala ʻokoʻa a hui pū me nā kaʻina ohi koʻikoʻi, hiki iā ia ke hāʻawi i kahi kumu hilinaʻi no ka maʻi maʻi. I ka wā e hiki mai ana, me ka hoʻomohala ʻana i ka lāʻau lapaʻau pololei, ʻoi aku ka koʻikoʻi o nā paipu hōʻiliʻili koko ʻo EDTA i ka nānā ʻana i ke koko, ka hoʻopili ʻana i nā gene a me nā ʻano ʻē aʻe, a hoʻomau i ka pale ʻana i ke olakino kanaka.